Part:BBa_K4231016
Y.lipolytica TEF1 promoter with a regulatory sequence
The length of A1 is 400 bp, the length of R1 is 200 bp, and the length of A3 is 338 bp. The three parts are assembled together to form the UAS sequence of the inducible TEF promoter. Among them, R1 sequence plays the role of oleic acid induction, A1 and A3 sequences play the role of enhancing transcription activity. Considering the limited length and capacity of upstream sequence, we choose a single-repeat R1 sequence to construct UAS sequence.
The promoter carrying this UAS can be strongly induced by oleic acid, and when the carbon source is glucose, the transcription activity is almost zero. This mixed promoter induced by oleic acid is nearly three times stronger than the native POX2 promoter and is a powerful tool for regulating and enhancing gene expression.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 797
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 797
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 797
- 1000COMPATIBLE WITH RFC[1000]
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